Trupti has over five years of experience studying the molecular basis of plant-microbe interactions (mainly on Arabidopsis thaliana and Pseudomonas syringae pathosystem). She trained in advanced molecular biology techniques – this involves techniques, including manipulation of DNA and RNA, cloning, bacterial and plant transformation and generation of transgenic plants expressing luciferase and GFP. Techniques she has used include cellular and sub-cellular localisation studies of the GFP/YFP lines by confocal microscopy, qRT-PCR to study gene expression, enumeration of pathogen symptoms, chlorophyll fluorescence imaging and quantification of fluorescence parameters, whole plant luciferase imaging and quantitation and her previous work has led her to discover which required the development of unique plant electrophysiology techniques.
During her Postdoc, she tried to develop image-based system for routine monitoring of plant health and early diagnosis of disease in the context of Cornish Agriculture. The ‘FIND (Fluorescence Imaging for Nutrition and Disease)’ project involved working closely with Cornish businesses.
Her role was to conduct routine diagnostic imaging of a range of plants (Basil, Tomato, Wheat, Barely, Rapeseed and Lettuce) during infection and nutritional stress in vertical farming applications. Particularly, she tried to investigate two well-defined plant imaging techniques; (1) Chlorophyll Fluorescence (CF) Imaging and (2) Normalised Difference Vegetative Index (NDVI).
Trupti's role at the MBA will be assisting with imaging Facility – organising the use of these facilities and helping to supervise staff and students who wish to use the facilities. Another aspect of her role will involve working directly on research projects related to Marine diatoms and Fungi.