Practical Course in Advanced Optical Microscopy
Dates for the 15th Course will be 4-14 April 2018.
Practical Course Location:
The Marine Biological Association
PL1 2PB U.K.
Tel: +44 (0)1752-426540
(The POSTER will be made available later in 2017)
The course takes place in the Laboratory of the Marine Biological Association from Wednesday 4 – Saturday 14 April 2018. Fee comprises teaching and full board; single room accommodation, cooked breakfast and lunch. Evening meals can be taken in local restaurants.
There will be 2–3 lectures each day throughout. Lectures will cover topics from basic optics, detectors and light sources through to photochemistry involved in the development of novel fluorescent probes and the application of advanced super-resolution optical microscopy techniques. The first 3 days will be spent setting up microscopes for brightfield and epifluorescence microscopy, including polarisation and interference contrast methods. The final 6 days will be organised in rotations around the 9 experiments listed below. The experiments will be in the large classroom at the MBA and in rooms nearby. Use will be made of marine specimens where possible and students may have an opportunity to trawl for benthos and plankton, depending on weather and availability of the MBA research vessel.
For an idea of the teaching schedule, please see the 2016 Timetable as example
Experiments with confocal imaging. CCD imaging and deconvolution, microinjection, genetically encoded probes, gene gun.
Confocal imaging with electrophysiology
CCD imaging with electrophysiology
Photolysis and photometry
Total internal reflection fluorescence imaging
Special demonstrations and hands-on exercises
- Multi-photon and confocal imaging
- Quantitative interference microscopy
- Use of an optical bench for basic experiments on scanning and apertures
- Spectroscopy and filters
- Abbe’s experiments on the role of diffraction in microscope image formation with absorption and phase objects.
Preparations used will include: invertebrate preparations (tunicates, molluscan neurones), zebra fish, mammalian brain slices, Fucus, Arabidopsis, and cell lines.
Students at the course will be invited to display a small poster of their current research and time will be allocated for presentation and discussion.
There is usually a 3:1 ratio of applications to places, and there will be up to 20 places on the course.
(APPLICATION FORM will be available later in 2017)
Please submit the Application Form by email, attaching your Curriculum Vitae and Research Project (giving a concise description of your research, your reasons for wishing to attend and your experience of techniques taught on the course) and PDF copy of a signed Letter of Recommendation on organisation letterheaded notepaper from an academic referee, preferably PhD supervisor or Head of Laboratory. This letter should indicate how your research, the laboratory in which you work and the area of research that you intend to pursue will benefit from your participation on the course.
Please make the subject of the email ‘Optical Course 2018′ followed by your surname.
Please send these three parts to arrive no later than close of office, 17:00 on 19 January 2018: email@example.com
All email submissions will be acknowledged. Applicants who are visa dependent will need to apply for tourist visas. Letters will be provided to those in need of supporting visa documentation.
There will be a registration fee for students accepted on the course. Accommodation, breakfast and lunches are included. Students who wish to request help towards fees should clearly indicate this in their application, and in their covering letter.
A very limited amount of funding for students’ travel costs will be available. Students who wish to claim travel expenses should clearly indicate this in their application.
Each day, there will be two lectures in the morning (probably 9.00 and 11.00), then practical work, and a lecture in the early evening, probably 6 pm.
Enquiries can be directed to firstname.lastname@example.org
Dr Brad Amos’ lecture at the Royal Society (45 mins) on revolutionary microscopy: http://royalsociety.org/events/2012/optical-microscope/ (click ‘Watch the video’)
- Instruments for Fluorescence Imaging
- Methods applicable to the study of both fresh and fixed materials
- Concepts for Imaging
- Odorant-evoked responses
- Detectors for single-molecule fluorescence imaging and spectroscopy
- Single-photon detectors for optical quantum information applications
- Single-photon generation and detection
- Ultraviolet and Visible Detectors for Future Space Astrophysics Missions
- Light Sheet Microscopy:
Basic opinions paper (Nature methods)
Light Sheet Microscopy Library (Jan Huisken)
OpenSPIM (including the Wiki)
http://openspim.org/EMBO_2014_final_program (click on recordings to watch the talks)