Algal signalling and physiology

Marine phytoplankton must constantly sense and respond to their dynamic environment in order to survive

Research Overview

We are interested in the cellular mechanisms in marine phytoplankton that underpin and drive global biogeochemical cycles. We study the mechanisms algae use to respond to their environment and use algae as model organisms to study fundamental signalling processes in eukaryotes.

We use a combination of genomic and physiological techniques in algae to examine novel cellular mechanisms responsible for membrane transport and signalling. Much of our research uses single cell microscopy approaches to visualise these processes directly. Some examples are shown below.

Current Research

Molecular mechanisms of calcification in coccolithophores

Coccolithophores are abundant bloom-forming phytoplankton that play an important role in the global carbon cycle due to their ability to produce calcium carbonate plates, known as coccoliths. Changes in the chemistry of our oceans caused by increased atmospheric CO2 may have a significant impact on coccolithophore calcification. In order to help predict how coccolithophores will respond to these rapid changes in their environment, we are examining how calcification is regulated at the cellular level and how these mechanisms may respond to environmental change.

We have recently made the surprising discovery that some coccolithophores require silicon in order to produce their coccoliths (Durak et al 2016). These species possess silicon transporters that are related to those found in extensively silicified phytoplankton, such as the diatoms. This finding has important implications for our understanding of the evolution of calcification and silicification by marine organisms. The absence of a requirement for silicon in bloom-forming coccolithophore species, such as Emiliania huxleyi, may have enhanced their ability to compete with the heavily silicified diatoms.

Calcium signalling in cilia and flagella

Cell biologists are becoming increasingly aware that cilia and flagella are important sensory organelles, which detect changes in the extracellular environment and convey these signals to the cell body. The biflagellate green alga, Chlamydomonas, is a model organism for the study of flagella function and has allowed researchers to link ciliary dysfunction to a range of human genetic disorders. We are using molecular, biochemical and cell physiological techniques to study signalling processes in Chlamydomonas flagella. We have developed techniques to image Ca2+ in both the cytosol and the flagella of Chlamydomonas and have recently demonstrated that intraflagellar Ca2+ elevations regulate the important process of intraflagellar transport (IFT) (Collingridge et al, 2013).

The evolution of ion channels

Eukaryote algae represent many diverse phylogenetic groups and therefore contain a wealth of genomic information relating to the evolution of fundamental cellular processes. Many ion channels associated with animal signalling processes are also present in the genomes of unicellular photosynthetic algae, including the voltage-gated Ca2+ channels, TRP channels and inositol triphosphate receptors (Wheeler and Brownlee, 2008). We are using comparative genomic approaches in combination with physiological studies to understand the evolutionary origins of these ion channels and their roles in algae. 

The evolution of algal metabolism

Algae represent many diverse photosynthetic eukaryotes with a complex evolutionary history. These lineages became photosynthetic when they engulfed a photosynthetic cyanobacterium or algal symbiont. This has resulted in a complex heritage of their genetic material and this complexity is also reflected in their physiology. We are interested in the processes and environmental pressures that have shaped algal evolution, as this will help us understand more about algae that are alive today.

Our early work demonstrated the pathway through which vitamin C is made in plants (Wheeler et al 1998). Plants and animals use different pathways to make vitamin C and a third pathway is found in the alga, Euglena. Our recent work has examined why these different pathways exist and identified a common trend. A number of animals (including primates, guinea pigs and some bats) have lost the ability to make vitamin C due to a defect in the final enzyme in the pathway (L-gulonolactone oxidase or GULO). Our research identified that plastid acquisition in plants and algae is also linked to the loss of GULO (Wheeler et al 2015). Plants and algae replaced GULO with an alternative enzyme, which may have helped to protect them from damaging reactive oxygen derived from the chloroplast.

Coccolithophore calcification: An unexpected requirement for silicon

Coccolithophores are well known for their ability to produce intricate protective scales composed of crystalline calcium carbonate (calcite). A collaborative project led by Dr. Glen Wheeler (MBA) is studying the unexpected finding that certain species of coccolithophores require silicon in order...

The role of ciliary calcium signalling in the regulation of intraflagellar transport

Cell biologists are becoming increasingly aware that cilia and flagella are important sensory organelles, which detect changes in the extracellular environment and convey these signals to the cell body. The biflagellate green alga, Chlamydomonas, is a model organism for the study of flagella...

Staff List

Glen Wheeler
Senior Research Fellow
Glen is a molecular cell biologist studying the physiology of marine phytoplankton and other algae.
Email: Telephone Number:
01752 426360
Katherine Helliwell
Post Doctoral Researcher
Email: Telephone Number:
+44(0)1752 426541
Gerald Langer
Research Associate
Gerald is interested in calcification of marine organisms, including the fundamental processes of calcification and its sensitivity to environmental factors such as temperature, nutrient availability and seawater carbonate chemistry.
Email: Telephone Number:
+44(0)1752 426360
Cecile Fort
Post Doctoral Researcher
Cecile finished her PhD in Philippe Bastin’s laboratory at the Pasteur Institut Paris, where she worked on trypanosomes and studied intraflagellar transport in the flagella of this parasite. During her PhD, she demonstrated that IFT is not essential for
Email: Telephone Number:
+44(0)1752 968703
Dorothee Kottmeier
Post Doctoral Researcher
Dorothee is postdoctoral researcher in Professor Brownlee's group investigating single cell physiology of coccolithophores and diatoms, using electrophysiological and imaging approaches for monitoring intracellular and cell surface properties. From
Email: Telephone Number:
+44(0)1752 968703
Abdesslam Chrachri
Postdoctoral Research Fellow
Abdul re-joined the MBA in 2007 and is working in various projects focusing on the cell biology of diatoms and coccolithophores, two of the most significant groups of phytoplankton with respect to ocean primary productivity and marine biogeochemical cycles
Email: Telephone Number:
+44(0)1752 426541
Susan Wharam
Research Assistant
Susie Wharam is a Molecular Biology Technician, within the Cellular and Molecular group. Susie obtained her PhD working on the molecular biology of plant-microbe interactions (Warwick University).
Email: Telephone Number:
+44(0)1752 426360
Serena Flori
Postdoctoral Research Associate
Serena Flori finished her PhD in the Cell & Plant Physiology Laboratory in Grenoble (France) in the frame of an ITN Marie Curie called AccliPhot.
Email: Telephone Number:
+44(0)1752 968703
Charlotte Walker
PhD Student
Charlotte is currently a NERC PhD Student based between the MBA and the University of Southampton National Oceanography Centre.
Email: Telephone Number:
+44(0)1752 426257
Friedrich Kleiner
PhD Student
Friedrich studied Biology at the Martin-Luther University of Halle-Wittenberg with the focus on insect and plant physiology.
Email: Telephone Number:
+44(0)1752 426257
Jack Dickenson
PhD Student
Jack is a PhD ('Cell signalling in Marine Phytoplankton in response to Environmental Stimuli') student , under the guidance of Professor Colin Brownlee (MBA), Dr Glen Wheeler (MBA) and Professor Mark Moore (Southampton).
Email: Telephone Number:
+44(0)1752 426330

2010-

  • Taylor AR, Brownlee C, Wheeler G (2017) Coccolithophore cell biology: chalking up progress. Ann Rev Mar Sci. 9:18.1–18.28.
  • Marron AO, Ratcliffe S, Wheeler GL, Goldstein RE, King N, Not F, de Vargas C, Richter DJ. (2016). The evolution of silicon transport in eukaryotes. Mol Biol Evol. 33(12):3226-3248.
  • Bickerton P, Sello S, Brownlee C, Pittman JK, Wheeler GL. (2016). Spatial and temporal specificity of Ca2+ signalling in Chlamydomonas reinhardtii in response to osmotic stress. New Phytol. 2016. doi: 10.1111/nph.14128.
  • Flynn KJ, Clark DR, Wheeler G. (2016). The role of coccolithophore calcification in bioengineering their environment. Proc Biol Sci. 283(1833). pii: 20161099.
  • Durak GM, Taylor AR, Walker CE, Probert I, de Vargas C, Audic S, Schroeder D, Brownlee C, Wheeler GL. (2016). A role for diatom-like silicon transporters in calcifying coccolithophores. Nature Commun. 7:10543.
  • Brownlee C, Wheeler GL, Taylor AR. (2015) Coccolithophore biomineralization: New questions, new answers. Semin Cell Dev Biol. 46:11-6.
  • Wheeler G, Ishikawa T, Pornsaksit V, Smirnoff N. (2015). Evolution of alternative biosynthetic pathways for vitamin C following plastid acquisition in photosynthetic eukaryotes. eLife 4:e06369.
  • Flynn KJ, Clark DR, Mitra A, Fabian H, Hansen PJ, Glibert PM, Wheeler GL, Stoecker DK, Blackford JC, Brownlee C. (2015). Ocean acidification with (de)eutrophication will alter future phytoplankton growth and succession. Proc Biol Sci. 282(1804):20142604.
  • Helliwell K. E., Collins S. Kazamia E. Purton S. Wheeler G. L. and Smith A.G. (2014). Fundamental shift in vitamin B12 eco-physiology of a model alga demonstrated by experimental evolution. The ISME Journal. 9(6):1446-55.
  • Keeling PJ, Burki F, Wilcox HM, Allam B, Allen EE, …Wheeler G… et al. (2014) The Marine Microbial Eukaryote Transcriptome Sequencing Project (MMETSP): Illuminating the Functional Diversity of Eukaryotic Life in the Oceans through Transcriptome Sequencing. PLoS Biology 12(6): e1001889
  • Collingridge P, Brownlee C, Wheeler GL. (2013) Compartmentalised calcium signalling in cilia regulates intraflagellar transport. Current Biology 23(22):2311-8
  • Read BA, Kegel J, Klute MJ, Kuo A, Lefebvre SC, Maumus F, Mayer C, Miller J, Monier A, Salamov A, Young J, Aguilar M, Claverie JM, Frickenhaus S, Gonzalez K, Herman EK, Lin YC, Napier J, Ogata H, Sarno AF, Shmutz J, Schroeder D, de Vargas C, Verret F, von Dassow P, Valentin K, Van de Peer Y, Wheeler G; Emiliania huxleyi Annotation Consortium, Dacks JB, Delwiche CF, Dyhrman ST, Glöckner G, John U, Richards T, Worden AZ, Zhang X, Grigoriev IV. (2013) Pan genome of the phytoplankton Emiliania underpins its global distribution. Nature. 499(7457), 209-13.
  • Helliwell KE, Wheeler GL, Smith AG. (2013). Widespread decay of vitamin-related pathways: coincidence or consequence?  Trends Genetics. 29(8):469-78.
  • Bach LT, Mackinder L, Schulz K, Wheeler GL, Schroeder DC, Brownlee C, Riebesell U. (2013) Dissecting the impact of CO2 and pH on the mechanisms of photosynthesis and calcification in the coccolithophore Emiliania huxleyiNew Phytologist. 199(1):121-34.
  • Flynn KJ, Blackford JC, Baird ME, Raven JA, Clark DR, Beardall J, Brownlee C, Fabian H, Wheeler GL. (2012) Changes in pH at the exterior surface of plankton with ocean acidification. Nature Climate Change. 2, 510-513
  • Chan CX, Zäuner S, Wheeler G, Grossman AR, Prochnik SE, Blouin NA, Zhuang Y, Benning C, Berg GM, Yarish C, Eriksen RL, Klein AS, Lin S, Levine I, Brawley SH, Bhattacharya D (2012). Analysis of Porphyra membrane transporters demonstrates gene transfer among photosynthetic eukaryotes and numerous sodium-coupled transport systems. Plant Physiol. 158(4):2001-12.
  • Taylor AR, Brownlee C, Wheeler GL. (2012). Proton channels in algae: reasons to be excited. Trends Plant Sci. 17(11):675-84.
  • Crawfurd KJ, Raven J, Wheeler GL, Baxter E, Joint I (2011). The response of Thalassiosira pseudonana to long-term exposure to increased CO2 and decreased pH. PLOS One. 6(10):e26695
  • Mackinder L, Wheeler GL, Schroeder DS, Von Dassow P, Riebesell U, Brownlee C. (2013) Expression of biomineralisation related ion transport genes in Emiliania huxleyiEnv Microbiol. 13(12):3250-65.
  • Helliwell KE, Wheeler GL, Leptos KC, Goldstein RE and Smith AG. (2011) Insights into the Evolution of Vitamin B12 Auxotrophy from Sequenced Algal Genomes. Mol Biol Evol. 28(10):2921-33.
  • *Taylor AR, *Chrachri A, *Wheeler GL, Goddard H and Brownlee C. A voltage-gated H+ channel underlying pH homeostasis in calcifying coccolithophores. PLOS Biology. 2011. 9(6):e1001085. (* denotes equal contribution).
  • Verret F, Taylor A, Wheeler G, Farnham G, Brownlee C. Calcium channels and their implications for evolution of calcium-based signalling in photosynthetic eukaryotes. New Phytologist. 2010. 187(1), 23-43.
  • Mackinder L, Wheeler G, Schroeder D, Riebesell U, Brownlee C. Molecular mechanisms underlying calcification in coccolithophores. Geomicrobiology. 2010. 27, 585-595.

1998-2009

  • Qudeimat E, Faltusz AM, Wheeler G, Lang D, Brownlee C, Reski R, Frank W. A PIIB-type Ca2+-ATPase is essential for stress adaptation in Physcomitrella patens. PNAS. 2008. 105(49) 19554-19559.
  • Wheeler GL, Brownlee C. Ca2+ signalling in plants and green algae – changing channels. Trends Plant Sci.2008. 13(9):506-14
  • Wheeler GL, Miranda-Saavedra D, Barton GJ. Genome Analysis of the Unicellular Green Alga Chlamydomonas reinhardtii Indicates an Ancient Evolutionary Origin for Key Pattern Recognition and Cell-Signaling Protein Families. Genetics. 2008. 179(1):193-7.
  • Wheeler GL, Joint I, Brownlee C. Rapid spatiotemporal patterning of cytosolic Ca2+ underlies flagellar excision in Chlamydomonas reinhardtii. Plant J. 2008. 53(3):401-13.
  • Thompson SE, Callow JA, Callow ME, Wheeler GL, Taylor AR, Brownlee C. Membrane recycling and calcium dynamics during settlement and adhesion of zoospores of the green alga Ulva linza. Plant Cell Environ. 2007. 30(6):733-44.
  • Joint I, Tait K, Wheeler G. Cross-kingdom signalling: exploitation of bacterial quorum sensing molecules by the green seaweed UlvaPhil Trans R Soc B. 2007. 362(1483):1223-33.
  • Conklin PL, Gatzek S, Wheeler GL, Dowdle J, Raymond MJ, Rolinski S, Isupov M, Littlechild JA, Smirnoff N. Arabidopsis thaliana VTC4 encodes L-galactose-1-P phosphatase, a plant ascorbic acid biosynthetic enzyme. J Biol Chem. 2006. 281(23):15662-70.
  • Bothwell JHF, Brownlee C, Hetherington AM, Ng CK, Wheeler GL, McAinsh MR. Biolistic delivery of Ca2+ dyes into plant and algal cells. Plant J. 2006. 46(2):327-35.
  • Wheeler GL, Tait K, Taylor A, Brownlee C, Joint I. Acyl-homoserine lactones modulate the settlement rate of zoospores of the marine alga Ulva intestinalis via a novel chemokinetic mechanism. Plant Cell Env. 2006. 29(4):608-18.
  • Wheeler GL, Grant CM. Regulation of redox homeostasis in the yeast Saccharomyces cerevisiaePhysiol. Plant. 2004 120(1):12-20.
  • Wheeler GL, Trotter EW, Dawes IW, Grant CM. Coupling of the transcriptional regulation of glutathione biosynthesis to the availability of glutathione and methionine via the Met4 and Yap1 transcription factors. J Biol Chem. 2003 278(50):49920-8.
  • Wheeler GL, Quinn KA, Perrone G, Dawes IW, Grant CM. Glutathione regulates the expression of gamma-glutamylcysteine synthetase via the Met4 transcription factor. Mol Microbiol. 2002. 46(2):545-56.
  • Collinson EJ, Wheeler GL, Garrido EO, Avery AM, Avery SV, Grant CM. The yeast glutaredoxins are active as glutathione peroxidases. J Biol Chem 2002. 277(19):16712-7.
  • Gatzek S, Wheeler GL, Smirnoff N. Antisense suppression of L-galactose dehydrogenase in Arabidopsis thaliana provides evidence for its role in ascorbate synthesis and reveals light modulated L-galactose synthesis. 2002. Plant J. 30(5):541-53.
  • Smirnoff N and Wheeler GL. Ascorbic acid in plants: biosynthesis and function. Crit Rev Biochem Mol Biol 2000. 35(4):291-314
  • Conklin PL, Norris SR, Wheeler GL, Williams EH, Smirnoff N and Last RL. Genetic evidence for the role of GDP-mannose in plant ascorbic acid (vitamin C) biosynthesis. PNAS 1999. 96:4198-4203
  • Wheeler GL, Jones MA and Smirnoff N. The biosynthetic pathway of vitamin C in higher plants. Nature 1998. 393:365-369

Ca2+ elevations in Chlamydomonas flagella viewed by TIRF microscopy

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Intraflagellar transport in Chlamydomonas during gliding motility

Dr Glen Wheeler

The Marine Biological Association of the United Kingdom,

The Laboratory, Citadel Hill,

Plymouth, Devon,UK.

PL1 2PB

..

Telephone: 01752 426360

Email: glw@mba.ac.uk