The seas are teeming with a huge diversity of life from the simplest single-celled organisms to complex animals.  The many different life forms that we find on the planet had their evolutionary roots in the oceans.  Life in the oceans also plays critical roles in the Earth's carbon and nutrient cycles and the regulation of climate.  The marine biota also provide a rich resource of models for the study of the evolution of fundamental life processes such as development and physiology as well as responses and adaptation to changes in their external environment.   Marine organisms also present a vast untapped resource for biotechnological advances.

Our research addresses mechanisms that underlie fundamental processes in algae, with particular focus on the marine phytoplankton, ranging from elucidating molecular mechanisms operating in single cells to the factors that regulate the dynamics of populations.  We work with population biologists and ecosystem modellers to better understanding how the phytoplankton drive ocean processes and respond and adapt to the changes in ocean chemistry that are occurring as a result of human activities on a global scale.

.

My research is currently funded by a European Research Council Advanced grant, the Natural Environment Research Council (NERC), Biotechnology and Biological Sciences Research Council (BBSRC), EU Horizon 2020 and the Gordon and Betty Moore Foundation.

.

I study algal cells in order to understand fundamental aspects of cell biology, including cellular transport, homeostasis and signalling. This research is providing unexpected insights into the evolution of membrane transport and signalling mechanisms in eukaryote organisms.  My research also addresses the cell biology of key marine organisms,  such as the calcifying coccolithophores and silicifying diatoms, that are of critical importance in global carbon and nutrient cycling. We adopt a multidisciplinary approach combining comparative physiology, molecular biology and genomic studies to better understand how phytoplankton populations may respond or adapt to changing conditions in the oceans.

.

The SeaCells Project

SeaCells addresses fundamental questions in phytoplankton biology from cellular to population scales.  This research is beginning to provide exciting new information on the mechanisms and evolution of membrane transport, cell signalling and metabolic regulation in phytoplankton species, such as diatoms and coccolithophores, that play important roles in driving biogeochemical cycles. The research builds on a number of recent findings, including the discovery of cell membrane properties that were thought to be typical of animal cells but now must be considered to be of much more ancient origin.   The 5-year SeaCells programme, funded by a European Research Council (ERC) Advanced Grant, brings together single cell biophysics, imaging and state of the art molecular biology with in situ studies of natural oceanic phytoplankton populations.  A major aim is to gain critical mechanistic understanding at the molecular and single cell level along with information on the microenvironment that surrounds cells.  In order to understand how the physiological properties of single cells in the laboratory translate to behaviour of natural populations we will transfer single cell technologies developed in the laboratory to ship-board platforms.  Knowledge of cell- to-cell variability will provide insights into the plasticity of populations and their responses to changing ocean conditions.  

.

How and why do coccolithophores calcify?

Coccolithophores are well known for their ability to produce intricate protective scales composed of crystalline calcium carbonate (calcite). Coccoliths are produced inside the cells and are secreted to the cell surface. We are interested in the mechanisms that underlie this incredible biological process, particularly the transport and regulatory mechanisms that bring about coccolith formation.  This knowledge is used to develop models of the costs and benefits of calcification that can be scaled from single cells to populations.

A collaborative project led by Dr. Glen Wheeler (MBA) is studying the unexpected finding that certain species of coccolithophores require silicon in order to correctly form their calcite scales.   Significantly, those species that do require silicon also possess diatom-like silicon transport genes that are associated with the process of silicification.  We wish to understand the evolutionary and ecological implications of this finding. This work has recently been funded by a joint NERC/NSF grant and will involve collaboration with Dr. Alison Taylor (University of North Carolina, Wilmington).

.

EMBRIC: The European Marine Biological Resource Centre Infrastructure Consortium 

EMBRIC is a Horizon 2020 Blue Growth project that brings together European research infrastructures and industry with the objectives of developing chains of services and pipelines for access to biological, analytical and data resources.  Our role is to work with European partners to develop protocols and pipelines for biotechnological applications with microalgae.

.

.

SEACELLS

The SeaCells project has been funded since October 2015 by a €2.7m European Research Council (ERC) Advanced Grant. SEACELLS addresses fundamental questions in phytoplankton biology from cellular to population scales.  Our recent studies of phytoplankton, primitive photosynthetic marine...

European Marine Biological Research Infrastructure Cluster to promote the Blue Bioeconomy (EMBRIC)

The Horizon 2020 NFRADEV-4-2014-2015 EMBRIC project is designed to accelerate the pace of scientific discovery and innovation from marine Bio-Resources. EMBRIC aims to promote new applications derived from marine organisms in fields such as drug discovery, novel foods and food...

 Scroll down for individual contact details

Abdul Chrachri is a postdoctoral cell physiologist.  Abdul works across several projects and provides electrophysiological and microscopical skills.  He records the electrical activity of phytoplankton cells using intracellular electrodes and patch clamp approaches. He also uses patch clamp electrophysiology to monitor the acitivity of specific ion channels from coccolithophores and diatoms that have been expressed in mammalian cell lines.

Jack Dickenson is a PhD student funded by the ERC SeaCells project. Jack studies cell biology and signalling in phytoplankton laboratory cultures and natural populations.

Serena Flori is a postdoctoral researcher who joined the ERC SeaCells group in December 2016. Serena will be developing imaging approaches for phytoplankton both in laboratory cultures and natural populations.  She has experience in diatom cell biology, cell physiology and microscopy.

Katherine Helliwell is a postdoctoral molecular biologist using molecular, genetic and cell imaging approaches to studying the cell biology and evolution of signalling in marine phytoplankton.  Katherine's current focus is on membrane ion channels and thier roles in diatoms and coccolithophores.  She is supported by the ERC SeaCells project.

Claire Jasper is a research technician, funded through the Horizon 2020 Blue Growth project EMBRIC.  Claire plays a key role in maintaining phytoplankton cultures for general research and for producing larger scale phytplankton cultures for biotechnological applications.

Friedrich Kleiner is a PhD student studying membrane biology and signalling in phytoplankton, supported by the ERC SeaCells project. Friedrich is using cell physiological, imaging and molecular approaches to understand how intracellular signals are generated in diatom cells.

Dorothee Kottmeier is a postdoctoral researcher working on the ERC SeaCells project.  Dorothee studies coccolithophore pH in relation to photosynthesis and calcification using a range of single cell physiological approaches.

Susie Wharam is a molecular biology technician working on the ERC SeaCells project.  Susie uses a range of molecular and genetic approaches for functional characterization of genes and molecules involved in signalling processes in diatoms and coccolithophores.

Angela Ward.  Cell and Molecular Biology laboratory manager.  Angela ensures that the laboratory functions smoothly!  She is also responsible for maintaining our research collection of phytoplankton cultures.

Cecile Fort.  Cecile is a postodctoral researcher studying calcium signalling mechanisms in relation to movement of protein molecules in the flagella of the single celled alga Chalmydomonas in a BBSRC-funded project led by Dr Glen Wheeler.

Gerald Langer is a postdoctoral researcher studying the mechanistic, ecological and evolutionary aspects of the requirement for silicon in coccolithophore calcification in a NERC-funded project led by Dr. Glen Wheeler.

Charlotte Walker is a NERC-funded PhD student studying the requirement for calcification in coccolithophore cell growth and division in collaboration with Dr. Glen Wheeler (MBA) and Professor Toby Tyrrell (University of Southampton).

Staff List

Katherine Helliwell
Post Doctoral Researcher
Email: Telephone Number:
+44(0)1752 426541
Charlotte Walker
PhD Student
Charlotte is currently a NERC PhD Student based between the MBA and the University of Southampton National Oceanography Centre.
Email: Telephone Number:
+44(0)1752 426257
Dorothee Kottmeier
Post Doctoral Researcher
Dorothee is a postdoctoral researcher in Professor Brownlee's group investigating single cell physiology of coccolithophores and diatoms.
Email: Telephone Number:
+44(0)1752 968703
Gerald Langer
Research Associate
Gerald is interested in calcification of marine organisms, including the fundamental processes of calcification and its sensitivity to environmental factors such as temperature, nutrient availability and seawater carbonate chemistry.
Email: Telephone Number:
+44(0)1752 426360
Serena Flori
Postdoctoral Research Associate
Serena Flori finished her PhD in the Cell & Plant Physiology Laboratory in Grenoble (France) in the frame of an ITN Marie Curie called AccliPhot.
Email: Telephone Number:
+44(0)1752 968703
Abdesslam Chrachri
Postdoctoral Research Fellow
Abdul re-joined the MBA in 2007 and is working in various projects focusing on the cell biology of diatoms and coccolithophores, two of the most significant groups of phytoplankton with respect to ocean primary productivity and marine biogeochemical cycles
Email: Telephone Number:
+44(0)1752 426541
Susan Wharam
Research Assistant
Susie Wharam is a Molecular Biology Technician, within the Cellular and Molecular group. Susie obtained her PhD working on the molecular biology of plant-microbe interactions (Warwick University).
Email: Telephone Number:
+44(0)1752 426541
Cecile Fort
Post Doctoral Researcher
Cecile finished her PhD in Philippe Bastin’s laboratory at the Pasteur Institut Paris, where she worked on trypanosomes and studied intraflagellar transport in the flagella of this parasite. During her PhD, she demonstrated that IFT is not essential for
Email: Telephone Number:
+44(0)1752 968703
Friedrich Kleiner
PhD Student
Friedrich studied Biology at the Martin-Luther University of Halle-Wittenberg with the focus on insect and plant physiology.
Email: Telephone Number:
+44(0)1752 426240
Jack Dickenson
PhD Student
Jack is a PhD ('Cell signalling in Marine Phytoplankton in response to Environmental Stimuli') student , under the guidance of Professor Colin Brownlee (MBA), Dr Glen Wheeler (MBA) and Professor Mark Moore (Southampton).
Email: Telephone Number:
+44(0)1752 426240

Brownlee selected recent publications

Professor Colin Brownlee

Director, Marine Biological Association

Chair of Marine Biosciences, School of Ocean and Earth Sciences, University of Southampton.

.

Address:

The Laboratory, Citadel Hill

Plymouth PL1 2PB

M: +44 7917 845471

T: +44 1752 426585 or 426274

cbr@mba.ac.uk